4TYE
Structural analysis of the human Fibroblast Growth Factor Receptor 4 Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.948, 61.948, 184.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.740 - 2.800 |
| R-factor | 0.22682 |
| Rwork | 0.223 |
| R-free | 0.27766 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | generated using Swissmodel |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.740 | 3.020 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.127 | 0.854 |
| Number of reflections | 9467 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 6.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.6 M NaH2PO4, 0.6 M KH2PO4, 0.075 M NaHEPES pH 7.5, 24% Glycerol |
