4TXI
Construct of MICAL-1 containing the monooxygenase and calponin homology domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-01 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.924, 49.885, 95.852 |
| Unit cell angles | 90.00, 97.03, 90.00 |
Refinement procedure
| Resolution | 32.142 - 2.309 |
| R-factor | 0.2193 |
| Rwork | 0.216 |
| R-free | 0.27930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.261 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.142 | 50.000 | 2.350 |
| High resolution limit [Å] | 2.309 | 6.270 | 2.310 |
| Rmerge | 0.079 | 0.033 | 0.493 |
| Total number of observations | 93863 | ||
| Number of reflections | 28540 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 95.1 | 98.7 | 73.8 |
| Redundancy | 3.3 | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 2000 MME, HEPES buffer |
