4TXD

Crystal structure of Thermofilum pendens Csc2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-23
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	80.951, 112.591, 60.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.218 - 1.818
R-factor	0.1831
Rwork	0.181
R-free	0.21210
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.102
Refinement software	PHENIX ((phenix.refine: 1.9_1678))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.220
High resolution limit [Å]	1.818
Number of reflections	50368
<I/σ(I)>	25.13
Completeness [%]	99.8
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	0.2 M KCl, 0.01 M MgCl2, 5% Peg 8000 and 17% glycerol