4TX8

Crystal Structure of a Family GH18 Chitinase from Chromobacterium violaceum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	120.480, 120.480, 106.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.670 - 2.170
R-factor	0.1692
Rwork	0.168
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ed7
RMSD bond length	0.003
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.880	53.880	2.230
High resolution limit [Å]	2.170	9.700	2.170
Rmerge	0.072	0.022	0.579
Number of reflections	40464
<I/σ(I)>	14.7		2.3
Completeness [%]	96.9	91.9	98.4
Redundancy	4.9	4.6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.7 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5