4TW5

Structure Of the First Two Thioredoxin Domains of Saccharomyces cerevisiae Eps1p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	P 1 21 1
Unit cell lengths	40.026, 133.031, 124.406
Unit cell angles	90.00, 92.00, 90.00

Refinement procedure

Resolution	41.767 - 2.370
R-factor	0.2153
Rwork	0.211
R-free	0.27160
RMSD bond length	0.010
RMSD bond angle	1.277
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.770	2.450
High resolution limit [Å]	2.370	2.370
Number of reflections	52451
<I/σ(I)>	8.7	2.9
Completeness [%]	99.4	99
Redundancy	4.9	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	24% PEG 4,000, 100 mM sodium acetate, pH 6.4, 25 mM sodium citrate, pH 6.4, 17% glycerol