4TW2
Crystal Structure of SCARB2 in Neural Condition (pH7.5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-13 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 0.91998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.796, 100.317, 105.173 |
| Unit cell angles | 90.00, 98.69, 90.00 |
Refinement procedure
| Resolution | 47.154 - 2.889 |
| R-factor | 0.2463 |
| Rwork | 0.244 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f7b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.369 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.105 | 0.059 | 0.535 |
| Number of reflections | 28574 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 99.1 | 98.6 | 93.1 |
| Redundancy | 3.7 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 mol/L HEPES (pH 7.5), 10% v/v 2-Propanol, 20% w/v Polyethylene glycol 4000 |
