4TW0

Crystal Structure of SCARB2 in Acidic Condition (pH4.8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-29
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.91998
Spacegroup name	P 1 21 1
Unit cell lengths	103.990, 99.636, 125.853
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	48.060 - 3.648
R-factor	0.2519
Rwork	0.249
R-free	0.30350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f7b
RMSD bond length	0.006
RMSD bond angle	1.281
Data reduction software	SCALEPACK
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.710
High resolution limit [Å]	3.648	9.880	3.650
Rmerge	0.141	0.040	0.707
Number of reflections	28909
<I/σ(I)>	6.5
Completeness [%]	98.4	96.4	98.1
Redundancy	2.9	2.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.2 mol/L ammonium sulfate, 0.1 mol/L sodium acetate trihydrate(pH 4.8), 30% w/v polyethylene glycol monomethyl ether 2000