4TVR
Tandem Tudor and PHD domains of UHRF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2822 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.280, 71.280, 120.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.290 |
| R-factor | 0.23067 |
| Rwork | 0.229 |
| R-free | 0.25982 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ask |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.973 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
| High resolution limit [Å] | 2.290 | 6.210 | 2.290 |
| Rmerge | 0.048 | 0.039 | 0.967 |
| Total number of observations | 120996 | ||
| Number of reflections | 14292 | ||
| <I/σ(I)> | 13.8 | ||
| Completeness [%] | 98.3 | 91.6 | 98 |
| Redundancy | 8.5 | 7.3 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 25% PEG-3350, 0.2 M NaCl, 0.1 M HEPES pH 7.5, 5% glycerol |
