4TVR

Tandem Tudor and PHD domains of UHRF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2822
Spacegroup name	P 43 21 2
Unit cell lengths	71.280, 71.280, 120.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.290
R-factor	0.23067
Rwork	0.229
R-free	0.25982
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ask
RMSD bond length	0.005
RMSD bond angle	0.973
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.290	6.210	2.290
Rmerge	0.048	0.039	0.967
Total number of observations	120996
Number of reflections	14292
<I/σ(I)>	13.8
Completeness [%]	98.3	91.6	98
Redundancy	8.5	7.3	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	25% PEG-3350, 0.2 M NaCl, 0.1 M HEPES pH 7.5, 5% glycerol