4TVM
Structure of Citrate Synthase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9725 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 148.767, 148.767, 182.797 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.140 - 2.600 |
| R-factor | 0.17494 |
| Rwork | 0.174 |
| R-free | 0.19405 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e6y |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.757 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.140 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 24114 |
| <I/σ(I)> | 7.91 |
| Completeness [%] | 99.9 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | Citrate synthase crystals grew at 20 degrees Celsius in 0.2M Magnesium chloride hexahydrate, 0.1M Tris pH=8.5, 3.4M 1,6-Hexandiol in a 75/25 protein/reservoir-ratio drop of 1 microlitre and with a protein concentration of 5.2 mg/ml. |
