4TVD
N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with D-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.770, 98.980, 181.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.700 - 2.300 |
| R-factor | 0.17155 |
| Rwork | 0.169 |
| R-free | 0.22332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ttq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.750 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | 0.650 |
| Number of reflections | 54664 | |
| <I/σ(I)> | 13.7 | 2.1 |
| Completeness [%] | 99.5 | 94.2 |
| Redundancy | 6.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 20% PEG 3350, 0.1 M ammonium nitrate |
