4TV0

Drosophila stem-loop binding protein complexed with histone mRNA stem-loop, Selenomethionine derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 41 3 2
Unit cell lengths	103.929, 103.929, 103.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.002 - 2.601
R-factor	0.253
Rwork	0.250
R-free	0.28450
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.157
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.099	0.650
Number of reflections	11133
<I/σ(I)>	26.7	2.6
Completeness [%]	99.8	99.5
Redundancy	23.8	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	16% (wt/vol) PEG3350, 0.2 M Ca(Ac)2, 50 mM cacodylic acid, pH 6.5