4TUQ

Human DNA polymerase beta inserting dCMPNPP opposite GG template (GG0b).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-05-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	50.820, 80.542, 55.535
Unit cell angles	90.00, 107.69, 90.00

Refinement procedure

Resolution	19.849 - 2.367
R-factor	0.2013
Rwork	0.198
R-free	0.25910
RMSD bond length	0.004
RMSD bond angle	1.044
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.367
Number of reflections	17394
<I/σ(I)>	15.7
Completeness [%]	100.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20% PEG3400, and 350 mM sodium acetate in 50 mM imidazole