4TUL
Crystal structure of monoclonal antibody against neuroblastoma associated antigen.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.676, 64.167, 78.900 |
| Unit cell angles | 90.00, 99.46, 90.00 |
Refinement procedure
| Resolution | 77.830 - 1.400 |
| R-factor | 0.1894 |
| Rwork | 0.188 |
| R-free | 0.21714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f8t |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.261 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.830 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.029 | 0.176 |
| Number of reflections | 81806 | |
| <I/σ(I)> | 14.52 | 3.98 |
| Completeness [%] | 99.0 | 99.87 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | MES, PEG 5000, NH4SO4 |
