4TUB

Crystal structure of tRNA-Thr bound to Codon ACC-C on the Ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	208.990, 444.571, 616.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.392 - 3.600
R-factor	0.2186
Rwork	0.217
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3I8F 3I8G 3I8H 3I8I
RMSD bond length	0.006
RMSD bond angle	1.002
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000	70.000	3.700
High resolution limit [Å]	3.600	50.000	3.600
Rmerge	0.187	0.014	0.905
Rmeas	0.220	0.017	1.064
Total number of observations	2161644
Number of reflections	757538	214	50291
<I/σ(I)>	7.3	46.26	1.65
Completeness [%]	96.8	68.6	97.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl