4TU0
CRYSTAL STRUCTURE OF CHIKUNGUNYA VIRUS NSP3 MACRO DOMAIN IN COMPLEX WITH A 2'-5' OLIGOADENYLATE TRIMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2008-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.86 |
| Spacegroup name | P 31 |
| Unit cell lengths | 87.371, 87.371, 84.662 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.670 - 2.300 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gpg |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.774 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.670 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 32115 | |
| <I/σ(I)> | 0 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 293 | 46% PEG 600, 100MM HEPES, PH 7.4, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
