4TTY

N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p G94D F145W Q147R (NN25)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	117.200, 44.220, 70.330
Unit cell angles	90.00, 117.40, 90.00

Refinement procedure

Resolution	40.697 - 1.900
R-factor	0.2115
Rwork	0.210
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q0y
RMSD bond length	0.003
RMSD bond angle	0.774
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.696	1.950
High resolution limit [Å]	1.900	1.900
Rmerge		1.390
Number of reflections	25239
<I/σ(I)>	14.83	1.66
Completeness [%]	98.4	93.8
Redundancy	7.5	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293	PEG3350, MES-NaOH