4TTU
N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with isomaltotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.290, 100.100, 184.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.990 - 2.180 |
| R-factor | 0.1687 |
| Rwork | 0.166 |
| R-free | 0.21305 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ttq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.662 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.111 | |
| Number of reflections | 66927 | |
| <I/σ(I)> | 11 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 15% PEG 3,350, 0.1M ammonium nitrate |
