4TT1

Crystal structure of fragment 1600-1733 of HSV1 UL36, native

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-14
Detector	PSI PILATUS 6M
Wavelength(s)	0.97911
Spacegroup name	P 61 2 2
Unit cell lengths	110.217, 110.217, 159.939
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.317 - 2.750
R-factor	0.2032
Rwork	0.201
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tt0
RMSD bond length	0.009
RMSD bond angle	1.140
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.317	2.750
High resolution limit [Å]	2.750	2.600
Number of reflections	15531
<I/σ(I)>	21	1.82
Completeness [%]	99.9
Redundancy	12.8	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200 mM diammonium hydrogenophosphate, 20% PEG 3350