4TSR
The Complex Structure of Mutant Phytase with IHS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.796, 47.500, 65.632 |
| Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.070 |
| R-factor | 0.17419 |
| Rwork | 0.172 |
| R-free | 0.21818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dkq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.792 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.070 |
| Rmerge | 0.129 |
| Number of reflections | 23694 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 99.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.01 M NiCl2, 0.1 M HEPES, 20% PEG 3350 and 2% PEG 8000 |
