4TSQ
Crystal structure of FraC with DHPC bound (crystal form III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 70.270, 70.270, 202.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.250 - 1.600 |
| R-factor | 0.13265 |
| Rwork | 0.132 |
| R-free | 0.15419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwi |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.146 |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.250 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.045 | 0.136 |
| Number of reflections | 141157 | |
| <I/σ(I)> | 17 | 5 |
| Completeness [%] | 95.4 | 77.1 |
| Redundancy | 3.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium thiocyanate, 20% PEG 3350, 30 mM DHPC, 3 mM dehydroepiandrosterone |
