4TSO
Crystal structure of FraC with DHPC bound (crystal form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 131.059, 131.059, 49.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.760 - 2.300 |
| R-factor | 0.18455 |
| Rwork | 0.182 |
| R-free | 0.22687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.572 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.760 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 | 0.500 |
| Number of reflections | 21326 | |
| <I/σ(I)> | 18.8 | 3.3 |
| Completeness [%] | 97.5 | 95.7 |
| Redundancy | 8.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 50 mM DHPC, 200 mM Li2SO4, 20% PEG 1000, 100 mM phosphate-citrate |
