4TSA
Structure of a lysozyme FAb complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.92000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.740, 97.570, 57.850 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 48.780 - 2.270 |
| R-factor | 0.2824 |
| Rwork | 0.281 |
| R-free | 0.30980 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 2.330 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.587 | |
| Number of reflections | 22427 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 97.8 | 91.8 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PCTP 100mM pH 6.5, 25% Peg10k |
