4TS7

Sulfolobus solfataricus adenine phosphoribosyltransferase with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	122
Detector technology	AREA DETECTOR
Collection date	2008-02-01
Detector	MARRESEARCH
Wavelength(s)	0.93300
Spacegroup name	P 61
Unit cell lengths	135.716, 135.716, 54.151
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.911 - 2.800
R-factor	0.1747
Rwork	0.172
R-free	0.22150
RMSD bond length	0.008
RMSD bond angle	1.151
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.145	0.465
Number of reflections	14393
<I/σ(I)>	18.3	5
Completeness [%]	100.0	99.9
Redundancy	8.6	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein 14 mg/mL 25 mM TRIS pH 7.6 0.1 mM EDTA 10 mM Mg(NO3)2 10 mM ATP Buffer 0.1 M bis-TRIS pH 5.5 Precipitant 21 % PEG3350 0.2 M Li2SO4