4TS5

Sulfolobus solfataricus adenine phosphoribosyltransferase with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	122
Detector technology	AREA DETECTOR
Collection date	2007-02-01
Detector	MARRESEARCH
Wavelength(s)	0.93100
Spacegroup name	P 61
Unit cell lengths	134.648, 134.648, 53.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.625 - 2.400
R-factor	0.1925
Rwork	0.190
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.060
Phasing software	PHENIX ((phenix.refine: 1.5_2))
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.489
Number of reflections	22064
<I/σ(I)>	21.7	3.4
Completeness [%]	99.9	99.7
Redundancy	7.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein 14 mg/mL 25 mM TRIS pH 7.6 0.1 mM EDTA 5 mM MgCl2 2mM AMP Buffer 0.1 M SPG pH 4.0 Precipitant 25 % PEG1500