4TRT

Deinococcus radiodurans DNA polymerase III subunit beta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97239
Spacegroup name	P 32 2 1
Unit cell lengths	84.410, 84.410, 198.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.2
Rwork	0.198
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pol
RMSD bond length	0.008
RMSD bond angle	1.255
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000		2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.045	0.014	0.528
Rmeas	0.053	0.017	0.623
Total number of observations	184746
Number of reflections	55287	672	4032
<I/σ(I)>	17.37	58.57	2.6
Completeness [%]	98.0	91.1	98.2
Redundancy	3.3		3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	21% PEG 5000 MME, 0.12 M TRIS, 3.6% HEXANEDIOL