4TRP

Crystal structure of monoclonal antibody against neuroblastoma associated antigen.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2013-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88563
Spacegroup name	P 1
Unit cell lengths	42.925, 46.925, 62.358
Unit cell angles	110.81, 92.80, 100.13

Refinement procedure

Resolution	42.914 - 1.250
R-factor	0.1508
Rwork	0.149
R-free	0.17790
Starting model (for MR)	1f8t
RMSD bond length	0.005
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.860	1.320
High resolution limit [Å]	1.250	1.250
Rmerge		0.457
Number of reflections	109662
<I/σ(I)>	10.99	2.71
Completeness [%]	89.1	86.7
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Hepes, PEG 4000, isopropanol