4TR8

Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	80.530, 92.410, 127.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.900 - 1.800
R-factor	0.2086
Rwork	0.207
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.110
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.900
High resolution limit [Å]	1.800
Number of reflections	88854
<I/σ(I)>	17.69
Completeness [%]	99.6
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M sodium acetate pH 4.5 and PEG 3350