4TR7
Crystal structure of DNA polymerase sliding clamp from Mycobaterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.490, 83.650, 84.030 |
| Unit cell angles | 63.58, 86.84, 85.20 |
Refinement procedure
| Resolution | 18.760 - 2.290 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.400 |
| High resolution limit [Å] | 2.290 |
| Number of reflections | 42571 |
| <I/σ(I)> | 19.53 |
| Completeness [%] | 96.4 |
| Redundancy | 3.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.2M sodium acetate trihydrate pH 7.0 and 20% PEG 3350 |
