4TQV
Crystal structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 115.245, 151.187, 162.406 |
| Unit cell angles | 68.66, 81.76, 90.10 |
Refinement procedure
| Resolution | 29.816 - 4.504 |
| R-factor | 0.28 |
| Rwork | 0.279 |
| R-free | 0.32010 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.120 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.580 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.597 | |
| Number of reflections | 57115 | |
| <I/σ(I)> | 14.7 | 1.3 |
| Completeness [%] | 96.2 | 91.4 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG4000, sodium potassium tartrate, N-(2-acetamido)iminodiacetic acid |
