4TQM
Structural basis of specific recognition of non-reducing terminal N-acetylglucosamine by an Agrocybe aegerita lection
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-01 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 54.230, 77.540, 89.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.313 - 2.000 |
| R-factor | 0.1928 |
| Rwork | 0.190 |
| R-free | 0.24170 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.758 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.469 | 46.313 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.093 | 0.383 | |
| Rmeas | 0.138 | ||
| Rpim | 0.054 | 0.041 | 0.237 |
| Total number of observations | 158592 | 5737 | 11674 |
| Number of reflections | 25714 | ||
| <I/σ(I)> | 10.2 | 19.1 | 2.9 |
| Completeness [%] | 98.8 | 99.8 | 92.1 |
| Redundancy | 6.2 | 6.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 2.3M ammonium sulfate, 0.1M sodium acetate pH 4.4 |
