4TQL

Computationally designed three helix bundle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999
Spacegroup name	P 1
Unit cell lengths	24.877, 41.547, 133.202
Unit cell angles	98.94, 92.25, 90.06

Refinement procedure

Resolution	43.826 - 2.800
Rwork	0.264
R-free	0.31000
RMSD bond length	0.013
RMSD bond angle	1.569
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	12173
<I/σ(I)>	14.3
Completeness [%]	94.2
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	0.1 M Tris HCl, 30% Jeffamine M-600