4TQJ

Structural basis of specific recognition of non-reducing terminal N-acetylglucosamine by an Agrocybe aegerita lection

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-01-01
Detector	RIGAKU RAXIS
Wavelength(s)	1.54187
Spacegroup name	P 21 21 21
Unit cell lengths	52.600, 111.700, 135.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.057 - 2.000
R-factor	0.1895
Rwork	0.187
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.050
Data reduction software	iMOSFLM (3.3.9)
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.310	49.057	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge		0.029	0.276
Rmeas	0.064
Rpim	0.027	0.014	0.181
Total number of observations	262862	12313	20303
Number of reflections	52439
<I/σ(I)>	16.8	35.1	3.8
Completeness [%]	95.7	99.8	87.9
Redundancy	5	6.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	30% PEG 8000 (w/v), 0.2M sodium acetate and 0.1M sodium cacodylate pH 6.4