4TQ7

N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p Q93E (NN10)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	46.050, 73.640, 94.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.435 - 2.643
R-factor	0.2102
Rwork	0.208
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q0y
RMSD bond length	0.002
RMSD bond angle	0.591
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.435	2.710
High resolution limit [Å]	2.643	2.640
Rmerge		0.537
Number of reflections	9912
<I/σ(I)>	25.06	6.26
Completeness [%]	99.7	97.1
Redundancy	12.5	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293	PEG3350, MES-NaOH