4TPZ

N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p F145W (NN2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	40.660, 97.630, 143.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.724 - 1.800
R-factor	0.1946
Rwork	0.193
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.092
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.724	1.850
High resolution limit [Å]	1.800	1.800
Rmerge		1.003
Number of reflections	53811
<I/σ(I)>	22.63	2.21
Completeness [%]	99.9	98.9
Redundancy	7.37	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	293	PEG6000, MES-NaOH