4TPS
Sporulation Inhibitor of DNA Replication, SirA, in complex with Domain I of DnaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.287, 34.685, 84.735 |
| Unit cell angles | 90.00, 102.09, 90.00 |
Refinement procedure
| Resolution | 82.860 - 1.650 |
| R-factor | 0.12968 |
| Rwork | 0.126 |
| R-free | 0.19293 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.670 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 82.860 |
| High resolution limit [Å] | 1.650 |
| Number of reflections | 52893 |
| <I/σ(I)> | 12.5 |
| Completeness [%] | 98.9 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM HEPES pH 7.5, 200 mM NH4 acetate, 25 % (w/v) PEG 3350, 1% (v/v) DMF |
