4TPL
West Nile Virus Non-structural protein 1 (NS1) Form 1 crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.74625 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 167.850, 167.850, 93.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.454 - 2.900 |
| R-factor | 0.1723 |
| Rwork | 0.170 |
| R-free | 0.20960 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.008 |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.454 | 2.960 | |
| High resolution limit [Å] | 2.900 | 7.880 | 2.900 |
| Rmerge | 0.313 | 0.094 | 9.000 |
| Rmeas | 0.315 | 0.095 | 9.091 |
| Total number of observations | 6267610 | ||
| Number of reflections | 33918 | 3270 | 3942 |
| <I/σ(I)> | 19.64 | 66.92 | 0.69 |
| Completeness [%] | 100.0 | 99.6 | 99.9 |
| Redundancy | 96 | 50 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 25% PEG 3000, 5% Glycerol, 20 mM Sodium Citrate pH 5.5 |
