4TOZ
MppA Periplasmic Murein Tripeptide Binding Protein, Unliganded Open Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.970, 76.810, 89.570 |
| Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| Rwork | 0.180 |
| R-free | 0.20550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rkm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.251 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 90.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 162196 |
| <I/σ(I)> | 38.5 |
| Completeness [%] | 99.6 |
| Redundancy | 1.008 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | drop contained 2 microliters of 10 mg/mL protein solution and 2 microliters of the reservoir solution containing 0.2 M sodium acetate trihydrate, 0.1 M Tris-HCl, pH 8.5, 30% w/v PEG 4000 |
