Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8088 |
| Spacegroup name | P 61 |
| Unit cell lengths | 136.262, 136.262, 90.735 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.650 - 2.351 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vo4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.304 |
| Data reduction software | XDS (December 2013) |
| Data scaling software | XSCALE (December 2013) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 2.490 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.084 | |
| Number of reflections | 39774 | |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | TRI-SODIUM CITRATE 1.2-1.3M IN HEPES BUFFER 0.1M, PH7.4 |
