4TNM
Crystal structure of Arabidopsis importin-alpha3 armadillo repeat domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | H 3 |
| Unit cell lengths | 127.518, 127.518, 89.782 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.660 - 2.900 |
| R-factor | 0.2207 |
| Rwork | 0.219 |
| R-free | 0.26260 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.780 |
| Data reduction software | iMOSFLM (1.0.7) |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.660 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.297 | |
| Number of reflections | 11858 | |
| <I/σ(I)> | 8.8 | 2.6 |
| Completeness [%] | 98.3 | 98.8 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Mg-acetate, 0.1 M MES pH 6.5, 19% PEG3350 |
