4TN0

Crystal Structure of the C-terminal Periplasmic Domain of Phosphoethanolamine Transferase EptC from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-04
Detector	NOIR-1
Wavelength(s)	1.2680
Spacegroup name	C 2 2 21
Unit cell lengths	121.850, 183.140, 121.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.580 - 2.400
R-factor	0.2168
Rwork	0.215
R-free	0.25630
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.284
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.000		2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.112	0.026	0.996
Rmeas	0.130	0.031	1.156
Total number of observations	392584
Number of reflections	102491	1142	7569
<I/σ(I)>	10.63	35.47	1.45
Completeness [%]	99.7	98.1	99.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	200 mM diammonium phosphate, 15% (w/v) PEG 3350