4TMY

CHEY FROM THERMOTOGA MARITIMA (MG-IV)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1995-11-13
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 43 21 2
Unit cell lengths	50.066, 50.066, 186.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.800
R-factor	0.179 *
Rwork	0.179
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tmy
RMSD bond length	0.013
RMSD bond angle	19.900 *
Data reduction software	SDMS ((DCREDUCE))
Data scaling software	SDMS ((SCALE))
Phasing software	AMoRE
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000	3.170
High resolution limit [Å]	2.800	2.800
Rmerge	0.037 *	0.079
Number of reflections	5381
<I/σ(I)>	13.6	6.9
Completeness [%]	95.0	91
Redundancy	3.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7		0.2M AMMONIUM SULFATE, 0.1M HEPES BUFFER (PH 7.0), 25% PEG 4000, 15MM MAGNESIUM CHLORIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir		15 (mM)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir	PEG4000	30 (%)