4TME

Crystal Structure of EutL from Clostridium Perfringens bound to ethanolamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	87.960, 87.960, 251.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.043 - 1.700
R-factor	0.1478
Rwork	0.147
R-free	0.16230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.240
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1525))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.043		1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.088	0.040	1.240
Rmeas	0.092	0.042	1.322
Total number of observations	1370706
Number of reflections	109437	1433	7950
<I/σ(I)>	17.1	41.84	1.79
Completeness [%]	100.0	100	100
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES buffer, 5% PEG 8000, 8% ethylene glycol, crystals were soaked in 20mM ethanolamine