4TM6

Crystal Structure of EutL from Clostridium Perfringens at 298K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	298
Detector technology	PIXEL
Collection date	2014-04-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	88.890, 88.890, 254.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.874 - 1.900
R-factor	0.1383
Rwork	0.137
R-free	0.15900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.131
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1555))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.874		1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.093	0.030	1.172
Rmeas	0.106	0.035	1.320
Total number of observations	357046
Number of reflections	79392	1062	5735
<I/σ(I)>	10.07	35.5	1.33
Completeness [%]	97.6	98.2	97.2
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES buffer, 5% PEG 8000, 8% ethylene glycol