4TLI
THERMOLYSIN (25% ISOPROPANOL SOAKED CRYSTALS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.140, 94.140, 130.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| Rwork | 0.164 |
| R-free | 0.20600 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.080 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.093 | 0.372 |
| Number of reflections | 24598 | |
| <I/σ(I)> | 5.9 | 2 |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 4.6MM THERMOLYSIN, 45% DMSO, 50MM TRIS-HCL, 2.5M CSCL, PH 7.5, pH 7.50 |
