4TL3
Mechanistic insights from the crystal structure of an inward proton-transporting Anabaena sensory rhodopsin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.265, 104.301, 111.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.190 - 2.300 |
| R-factor | 0.22137 |
| Rwork | 0.220 |
| R-free | 0.24951 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xio |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.203 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.190 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 28486 | |
| <I/σ(I)> | 11.4 | 2 |
| Completeness [%] | 96.7 | 98.7 |
| Redundancy | 1.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 293 | MPD, pH 5.6, magnesium chloride |
