4TKT

Streptomyces platensis isomigrastatin ketosynthase domain MgsF KS6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97913
Spacegroup name	P 32 2 1
Unit cell lengths	83.331, 83.331, 203.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.612 - 2.429
R-factor	0.1854
Rwork	0.183
R-free	0.23780
RMSD bond length	0.003
RMSD bond angle	0.655
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.470
High resolution limit [Å]	2.429	6.590	2.429
Rmerge	0.079	0.039	0.983
Total number of observations	326286
Number of reflections	31725
<I/σ(I)>	8.2
Completeness [%]	99.8	97	100
Redundancy	10.3	9.5	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	297	1.6M Magnesium sulfate, 0.1M MES pH 6.5