4TJX
Crystal structure of protease-associated domain of Arabidopsis VSR1 in complex with aleurain peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-12-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.513, 58.562, 36.583 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 36.580 - 1.902 |
| R-factor | 0.1591 |
| Rwork | 0.154 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tjv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.040 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.580 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.035 | 0.134 |
| Number of reflections | 10308 | |
| <I/σ(I)> | 21.5 | 7.6 |
| Completeness [%] | 94.1 | 85.4 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M HEPES pH 7.0, 25% PEG 6000 |
