4TJV

Crystal structure of protease-associated domain of Arabidopsis vacuolar sorting receptor 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2011-08-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	32.600, 62.680, 35.810
Unit cell angles	90.00, 109.39, 90.00

Refinement procedure

Resolution	33.780 - 1.651
R-factor	0.1675
Rwork	0.166
R-free	0.19020
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.352
Data scaling software	XDS
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.780	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.026	0.148
Number of reflections	15984
<I/σ(I)>	74	19.1
Completeness [%]	97.7	94.9
Redundancy	15.5	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	10% glycerol, 0.1 M MES pH 6.0, 30% PEG 600, 5% (w/v) PEG 1000.