4S3Q
Amylomaltase MalQ from Escherichia coli in complex with maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.132, 77.019, 128.403 |
| Unit cell angles | 76.11, 75.48, 66.31 |
Refinement procedure
| Resolution | 34.230 - 2.100 |
| R-factor | 0.2227 |
| Rwork | 0.221 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4s3r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.275 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 10.000 | 2.100 |
| Rmerge | 0.058 | 0.020 | 0.394 |
| Number of reflections | 138781 | 1118 | 18524 |
| <I/σ(I)> | 12.02 | 33.34 | 3.03 |
| Completeness [%] | 94.3 | 81.7 | 96.4 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 20 %(w/v) PEG3350, 200 mM NaH2PO4, 250 mM NaCl, 20 mM Tris-HCl, protein concentration 13 mg/ml, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
