4S3H
Crystal structure of S. pombe Mdb1 FHA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-11 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 91.073, 91.073, 224.503 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.759 - 2.701 |
| R-factor | 0.2211 |
| Rwork | 0.218 |
| R-free | 0.27070 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.770 | 2.700 |
| Rmerge | 0.102 | 0.040 | 0.673 |
| Number of reflections | 15871 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.9 | 99.9 | 99.8 |
| Redundancy | 9.6 | 8.6 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 0.1 M sodium acetate, pH 5.2, 1.8 M ammonium sulfate, vapor diffusion, hanging drop, temperature 293K |
